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Overview of laboratory services

All columns marked with (Gelber Punkt) indicate methods used by the SPIEZ LABORATORY

Overview of special services in bacteriology

Detection of
risk group 3 bacteria 		CULTURE IMMUNO MOLBIO MS
Bacillus anthracis Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Yersinia pestis Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Francisella tularensis Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Borrelia burgdorferi Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Burkholderia mallei Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Burkholderia pseudomallei Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Brucella spp. Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Coxiella burnetti     Gelber Punkt  
Rickettsia prowazekii     Gelber Punkt  

Abbreviations
CULTURE Growing bacteria on solid and/or liquid culture media, poss. including microscopy and biochemical tests
IMMUNO Immunological detection: Different methods and platforms
MOLBIO Molecular biological detection: Different methods and platforms
MS Mass spectrometric detection: MALDI TOF (Matrix-Assisted Laser Desorption Ionisation Time-Of-Flight)

 

Detection of the following viruses possible IF ELISA NT PCR SEQ VI
Chikungunya Gelber Punkt Gelber Punkt   Gelber Punkt   Gelber Punkt
Dengue 1-4   Gelber Punkt   Gelber Punkt   Gelber Punkt
Hanta (Puumala, Dobrova, Seoul) Gelber Punkt Gelber Punkt   Gelber Punkt   Gelber Punkt
Influenza A (H1N1, H5N1)       Gelber Punkt   Gelber Punkt
Japanese encephalitis       Gelber Punkt   Gelber Punkt
Monkeypox       Gelber Punkt Gelber Punkt  
Norovirus       Gelber Punkt    
O' Nyong Nyong       Gelber Punkt   Gelber Punkt
Orthopox   Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
Polio 1-3       Gelber Punkt   Gelber Punkt
Rift Valley       Gelber Punkt    
St. Louis encephalitis       Gelber Punkt   Gelber Punkt
Tick-borne encephalitis (TBE) Gelber Punkt Gelber Punkt   Gelber Punkt   Gelber Punkt
Vaccinia   Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt
West-Nile Gelber Punkt Gelber Punkt   Gelber Punkt   Gelber Punkt
Yellow fever   Gelber Punkt Gelber Punkt Gelber Punkt   Gelber Punkt

Abbreviations:
IF Immunofluorescence
ELISA Enzyme Linked Immunosorbent Assay
NT Neutralisation test
PCR Polymerase chain reaction
SEQ Sequencing
VI Virus Isolation in cell cultures
Detection of the following
toxins is possible 		LFA ELISA SAS GE BA MS HPLC GC-MS
Ricin Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt    
Botulinumneurotoxine A,B,E+F Gelber Punkt Gelber Punkt   Gelber Punkt Gelber Punkt    
Staphylococcal
Enterotoxin B		 Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt Gelber Punkt    
Saxitoxin (PSP) Gelber Punkt Gelber Punkt       Gelber Punkt Gelber Punkt  
Domoic acid (ASP)             Gelber Punkt  
Ricinin (Ricin Marker)           Gelber Punkt Gelber Punkt Gelber Punkt
L-Abrin (Abrin Marker)           Gelber Punkt Gelber Punkt  

Abbreviations
LFA Lateral Flow Assay
ELISA Enzyme Linked Immunosorbent Assay
SAS Suspension Array System (BioPlex-200, Bio-Rad)
GE Gelelektrophoresis (Western-Blot) + Bioanalyzer 2100
BA Bioassay (in vitro + in vivo)
MS Mass Spectrometry (LC-MS, MALDI-TOF)
HPLC High Pressure Liquid Chromatography (mit Fluorescence Detektion)
GC-MS Gas Chromatography with MS Detektion
(*) Mouse-Assay (External at Miprolab D-Göttingen)
Determination of values in human blood possible PM
Cholinesterase (Serum- and acetylcholinesterase)* Gelber Punkt

* STS 054 accreditation
Abbreviations: PM = photometric